Ender VX-500

This page provides the model files of an open source 3D-Printer RNA extraction apparatus named the "Ender VX-500". This machine runs a semi-automated workflow for paramagnetic bead-based extraction of SARS-CoV-2 RNA from upper respiratory samples using readily accessible reagents. This innovation can deliver high-performance, low-cost and rapid molecular diagnostics for COVID-19.

The platform essentially consists of a Creality3D Ender-3 printer repurposed by installing three different custom designed interlocking attachments on the printer's extruder mounting mechanism. A 3D print “job” can be executed on the printer which will emulate the movements required to make the magnetic combs on the mounted attachments run a paramagnetic bead-based RNA extraction protocol. The resulting RNA from the platform is amenable to any downstream molecular applications including RT-qPCR, LAMP or genome sequencing.

A single instrument run by one operator can process 72 specimens per hour. In high-